This apparatus has been designed for the breaking down of both animal and bacterial tissues and for mixing operations which, in many instances, furnishes results superior to those obtained by ultrasonic equipment. Active enzyme preparations can be obtained with the Disintegrator from various kinds of yeast. The yeast Torulopsis Pulcherina can be broken down in 15 minutes and bacterium coli suspensions undergo 80% disintegration in approximately 25 minutes. Streptococcus faecalis and Staphylococcus aureus have been effectively disrupted by grinding with ballotini beads. The apparatus has also been used for the grinding of algae, fungi, “creaming” of rat’s livers, obtaining preparations of isolated nuclei, etc. Also for the extraction of DNA, even with difficult microorganisms such as small Gram-positive cocci.
The grinding or colloidizing action is caused by the violent impulses submitted to the test material as the reeds to which the sample containers are attached, reverse their direction 100 times per second (120 for 60 hz model). Two 10 cc capacity ‘Pyrex’ glass specimen vessels are held in stainless steel containers which are partly open so that the specimen can be observed during the disintegration process. Mounting the specimen holders in the plane of vibration of the reeds, and inwardly protruding baffles in the walls of the vessels themselves, prevent the material from clinging to the sides of the latter. This arrangement also avoids the formation of vortices, which would disturb the rapid and thorough mixing or disintegration. The disintegrator can also be supplied with containers to hold ten vials instead of the two ‘Pyrex’ vessels.